Enhanced ELISA using a handheld pH meter and enzyme-coated microparticles for the portable, sensitive detection of proteins.

نویسندگان

  • Yun Zhang
  • Jiani Yang
  • Jinfang Nie
  • Juanhua Yang
  • Dong Gao
  • Lang Zhang
  • Jianping Li
چکیده

This work describes a general methodology for enhanced enzyme-linked immunosorbent assay (ELISA) that integrates enzyme-coated microparticle probes for robust yet highly efficient signal amplification and a handheld pH meter for a simple, portable, quantitative readout. Its utility is well demonstrated with the detection of the target protein with a 14-fold enhancement of sensitivity in comparison with the conventional optical ELISA.

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عنوان ژورنال:
  • Chemical communications

دوره 52 17  شماره 

صفحات  -

تاریخ انتشار 2016